FastQCFastQC Report
Thu 28 Apr 2016
ERR1042039.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042039.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4570917
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT162000.35441466121568166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT136350.29829900652319874No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT127180.27823738650253327No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51510.11269073579765285No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA48690.10652129539871322No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC46300.10129258527337075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59500.018.9663871
TCTAGCG6850.016.2043828
TCGTTTA7950.015.59119430
ACGGACC10350.015.5507248
GACGGAC10250.015.3414637
AAGACGG11100.015.3333335
CTAGCGG7200.015.15972229
TAACGCC9250.014.84
CGAACGA4900.014.72448916
CGCAATA8000.014.5687536
TCTAACG908.28066E-414.3888882
TTCGGAA10650.014.24413331
GTATTAG12300.014.1382111
TTTCGGA10900.014.08715530
ATAACGC9800.013.9693873
CGCAAGA12000.013.7208342
TATACTG10400.013.6971145
CAAGACG13000.013.6615394
CGTTTAT9250.013.631
GTACATG128200.013.5791741
Produced by FastQC (version 0.11.5)