FastQCFastQC Report
Thu 28 Apr 2016
ERR1042038.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042038.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5978337
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT95020.15894052141925086No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74930.125335858450268No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73410.12279334537347092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38250.019.3464051
TCTAGCG8050.016.08695628
TCGTTTA6150.015.64227630
TACGACG8250.015.4727285
CGACGGT8000.015.2625017
ACGACGG8300.015.1566276
CGTATTA3000.014.815
ACGGACC11600.014.6724158
GACGGAC11750.014.6425537
CTAGCGG8750.014.37714329
TAATCGG2600.014.23076916
AAGACGG12700.013.9842525
TAAACGC6150.013.83739928
GACGGTA8700.013.8218398
CGAACGA5150.013.65048616
CGCAATA9500.013.242105536
GTATCAA55450.013.1451752
TAACGCC8350.013.07185654
TTAGACT5950.013.0588244
ATCCGTA3400.013.05882412
Produced by FastQC (version 0.11.5)