FastQCFastQC Report
Thu 28 Apr 2016
ERR1042036.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042036.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2106797
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44760.21245521044505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43710.20747134156731759No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32050.1521266643155463No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT24210.11491377669514433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGC450.003826850320.55555514
CTAGCGG4100.019.40243729
CGCAATA4300.018.93023336
GTACGAC701.2198092E-418.53
TCTAGCG4450.017.87640628
CCGGTCC5200.016.3653839
TACCGTC4450.016.2134847
CGGTCCA4950.016.07070710
ACGAACG2550.015.96078415
GACGGAC6100.015.7704937
CGAACGA2600.015.65384516
TATACCG1302.5917507E-715.6538455
GTATTAG5700.015.5789481
ACGGACC6100.015.4672148
AAGACGG6350.015.4409455
GTAAACG3750.015.29333427
GCGCAAT5450.015.27522935
CGCAAGA6950.015.1726622
TAAACGC3800.015.09210628
TAGCGGC5400.015.07407530
Produced by FastQC (version 0.11.5)