FastQCFastQC Report
Thu 28 Apr 2016
ERR1042035.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042035.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517127
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17850.1176565969757311No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16060.10585798024819279No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT15200.10018937109417998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10400.019.0336531
TCTATAC1757.2759576E-1216.9142863
GTATTAA2100.016.7380941
TAAACGC1554.0199666E-1016.70967928
GTAAACG1452.9813236E-916.58620627
CGTCCTA803.3834574E-416.187533
CGCAAGA3550.015.6338022
TACCGTC2850.015.5789477
TAATACC2500.015.544
CGTCGTA2650.015.35849110
ACCGTCG2650.015.3584918
CAAGACG4100.015.3414634
GATCCGT855.3655554E-415.23529429
TACTCGC855.3655554E-415.23529420
AAGACGG3650.015.2054815
TCTAGAC2201.8189894E-1215.1363643
CGGCGAT4000.014.830
ACGCTTC2251.8189894E-1214.831
CCGTCGT2750.014.7999999
ATACCAT2500.014.7999996
Produced by FastQC (version 0.11.5)