FastQCFastQC Report
Thu 28 Apr 2016
ERR1042034.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042034.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719364
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC8190.11385056800173486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA502.7183887E-729.61
TAACGAA1054.0017767E-1122.90476413
ATAACGA1107.4578566E-1121.86363612
GGTATCA4500.021.3777771
CGAACGA1151.3460522E-1020.91304416
ATGTGCG450.003824437520.5555557
CAACCCG450.003824437520.55555510
ACCGCGT555.1405246E-420.1818188
AACGAAC1202.382876E-1020.04166614
GTATAGA851.2439832E-619.5882341
CGCAATA951.6739432E-719.47368436
AACGTCG500.00703238518.527
GCTATAC801.6153004E-518.53
TAGGGGT1103.8457074E-818.54
GTCTTAG902.1499382E-618.51
CGTTTAT1002.871202E-718.531
GACTCCG609.232237E-418.57
TCTATAC500.00703238518.53
AATTCCG1451.546141E-1017.8620685
ACGAACG1351.1496013E-917.81481615
Produced by FastQC (version 0.11.5)