FastQCFastQC Report
Thu 28 Apr 2016
ERR1042032.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042032.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6998276
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT97970.139991620793464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT75740.10822665467895234No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT73820.10548312184315109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49500.020.6303041
GACGGAC12900.017.0658917
ACGGACC13400.016.0149258
AAGACGG15450.015.6860855
AGACGGA15200.014.9703956
TCGTTTA10200.014.87254930
GTATCAA70750.014.4600712
TATACTG12550.014.4462155
TATACCG3100.014.322585
GTACTAG4800.014.2604161
CTAGTAC5200.014.2307683
CGGACCA15450.013.8899699
TCTATAC10000.013.8753
CTAGCGG9850.013.52284329
ATAACGC12100.013.4545453
TAACGCC12000.013.4124994
TCTAGCG9950.013.38693428
TTAGACT6500.013.3769234
CGTTTAT11700.013.12393131
CGCAAGA16400.012.9725622
Produced by FastQC (version 0.11.5)