FastQCFastQC Report
Thu 28 Apr 2016
ERR1042031.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042031.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5528288
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT239170.4326294143865153No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT198760.3595326437407024No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT170240.3079434356531353No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72450.1310532302224486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAGA11950.016.1004182
ACGGACC11700.015.3376078
AAGACGG13150.015.3346025
GTACTAG4000.015.2625011
TCTATAC8650.015.1849713
CTAGCGG8350.015.06586829
TCTAGCG8300.014.93373528
GACGGAC12200.014.8606567
CTACGCT1001.0945611E-414.84
CGAACGA5600.014.20535716
CGGACCA12800.014.16406259
TACCGTC9000.013.97777757
GGTATCA109300.013.96386051
AGACGGA13500.013.840746
TTAACGG3000.013.56666735
TATACTG9850.013.5228415
CGTCGTA7950.013.49685510
CGCAATA9350.013.45454636
TCGTTTA8850.013.378530530
ATACCGT11450.013.2489086
Produced by FastQC (version 0.11.5)