FastQCFastQC Report
Thu 28 Apr 2016
ERR1042029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6235009
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT306710.4919158897765825No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT239150.3835599916535806No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT223540.35852394118436715No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98970.1587327299768132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86750.025.142941
CTAGCGG6950.016.76978529
GTATCAA133600.016.2982792
TCTAGCG7250.016.07586328
CGCAAGA11750.015.1148942
TATACTG13200.014.8560625
GACGGAC11100.014.8333337
ACGGACC11000.014.6318188
ATACGAC1551.2128294E-714.322583
AAGACGG12800.014.3085945
CGCAATA8150.014.30061336
CGGACCA11650.014.1330479
TTAACGG4400.013.45454535
GTATAGG13200.013.1742431
GTGTAAG10000.013.1349991
CGAGCCG10150.012.940886515
CGACGGT6600.012.893947
GCGCAAG13350.012.74906351
GTACTAG4500.012.7444451
TGCGACG1602.7002407E-612.7187522
Produced by FastQC (version 0.11.5)