FastQCFastQC Report
Thu 28 Apr 2016
ERR1042027.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042027.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2642012
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT365751.3843616153143892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT277641.0508657795649679No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT251370.9514339828887984No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120240.4551076982239294No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59020.22339035553207176No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52230.19769024516164196No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41260.15616885918761914No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35380.13391309350600983No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34880.13202059642424033No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG27420.10378453996423938No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27010.10223269235718839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG509.096928E-625.89999822
TAACGGA551.9038724E-523.54545428
CGTTAGA903.8344297E-922.6111132
GGTATCA104900.021.762631
CTAGCGT450.003827102720.5555578
CGTAGCG2900.020.41379415
GGCGTCG801.6179654E-518.58
GCGTTAG701.2199582E-418.51
ACGGACC500.00703724418.4999988
CGTACGA500.00703724418.49999812
TTTAGCG3950.018.26582326
TCGTAGC3250.018.21538514
GTGACAC5400.018.15740624
TTAACGG5100.018.13725535
TAACGGC5150.017.96116436
CGGGGTG3750.017.75999831
GGCACCG5650.017.6814169
CAATGCG752.0685463E-417.26666819
GTACGAG650.001580668617.07692313
ATTAGAG6100.016.9836063
Produced by FastQC (version 0.11.5)