FastQCFastQC Report
Thu 28 Apr 2016
ERR1042026.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042026.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14088900
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT650170.4614767653968727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT473860.33633569689613807No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT460350.3267465877392841No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186290.13222465912881773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA180350.024.4854471
GTATCAA275800.015.9376352
TTAACGG9650.014.95336835
TAACGGC9850.014.64974536
ACGGACC14950.014.478268
CTAGCGG10750.013.93953429
GACGGAC15900.013.8459117
TCTAGCG11050.013.72850728
GTATTAG39600.013.2676771
TCTATAC21050.013.0071273
AAGACGG19300.012.9404145
TATACTG24450.012.9386495
TACGACG11700.012.6495735
CGGACCA16900.012.5887579
GTATAGG27800.012.5773381
GTACTAG9500.012.4631581
AACGCAG354450.012.4116247
CGAACGA9900.012.33333416
CGCAAGA19900.012.2713572
ATCAACG358300.012.2576064
Produced by FastQC (version 0.11.5)