FastQCFastQC Report
Thu 28 Apr 2016
ERR1042025.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042025.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2839594
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT337891.189923629927377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT254540.896395752350512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT236340.8323020826216705No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89940.3167354206270333No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33790.11899588462294258No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30260.10656452999971122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104450.020.5280041
TGCGACG851.246628E-619.58823622
GACGGAC2000.017.5757
CGTAGCG1401.873559E-917.17857215
TCTAGCG2050.017.14634128
CGACGGT1851.8189894E-1116.0000027
TCACGTA2200.015.97727325
CGCAATA2350.015.74467936
GTCTAGT2003.6379788E-1215.7251
CGATAAC1701.4897523E-915.23529510
TAATACG1353.9778206E-715.0740754
GTATTAG5650.015.0619471
GTACTAG2251.8189894E-1214.81
TAGCGGA1651.6480044E-814.57575717
CGGACCA2700.014.3888899
TAATACT6300.014.3888884
TCGTAGC1803.3360266E-914.38888814
CTAGCGG2450.014.34693829
GTATCAA150500.014.2591352
TTAACGG1956.7484507E-1014.23076835
Produced by FastQC (version 0.11.5)