FastQCFastQC Report
Thu 28 Apr 2016
ERR1042024.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042024.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5249396
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT551211.0500446146566196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT399660.761344733756036No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT389780.7425235208012503No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165700.3156553630170023No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67710.12898626813446729No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58300.11106039628178176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129000.028.1372091
CGCGAAA450.003827598920.55555715
TCGACGC609.242802E-418.54
TCGTAGC4400.018.07954614
GTATCAA201350.018.0451972
TCTAGCG2700.017.1296328
TAGCGGA4800.016.57291617
GCGGAAT4850.016.40206119
CGTAGCG4950.016.07070715
CTAGCGG2900.015.94827629
CGCTATA1101.453752E-515.1363642
GCGTTAC1601.0990334E-815.0312514
CGACGCA750.004107001214.7999995
TCTGTCG6300.014.0952398
GTCGTAG6650.013.90977413
TATACTG10350.013.7632865
CGCAACG950.001246109813.63157912
AATCGGG6150.013.53658523
ATCAACG273200.013.2723284
TAGGACA7300.013.1780824
Produced by FastQC (version 0.11.5)