FastQCFastQC Report
Thu 28 Apr 2016
ERR1042023.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042023.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4409084
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT456071.0343871879056965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT334820.7593867569771863No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT314580.7134815304040476No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133140.30196748349543806No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57430.13025381235648947No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47740.10827645833012027No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC47230.10711975548662715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC9200.019.3043487
ACGGACC9350.018.9946528
TATAGCG801.6183663E-518.57
CGGACCA9850.018.4060929
AAGACGG10050.017.8557225
CAAGACG10400.017.6105794
GGTATCA174750.016.7902721
CTTACAC4950.016.4444453
GCGCAAG11600.016.1077581
TCTAGCG5500.015.80909128
CGCAAGA11550.015.696972
CGAACGA5200.015.65384816
GCGAAAG11250.015.62222318
AGACGGA11400.015.5789486
CTACGCT1201.9384352E-615.4166674
CGAAAGC11050.015.40271519
GTCGTAC855.368437E-415.2352941
GAGCGAA11700.015.02136816
CGATAAC5450.014.9357810
CTAGCGG5850.014.86324829
Produced by FastQC (version 0.11.5)