FastQCFastQC Report
Thu 28 Apr 2016
ERR1042022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3313566
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT265130.800134960341819No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT200280.6044243573238016No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT183700.554387629520583No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80930.24423838245563845No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39180.11824119392823321No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36860.11123967351186004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94650.019.838881
CGCAAGA6500.017.0769232
GACGGAC6150.016.8455287
AAGACGG6950.016.5035975
ACGGACC6400.015.89843758
GAACCGT1455.3551048E-815.3103456
CTAGCGG3550.015.11267629
CGTAGCG1353.978421E-715.07407515
GCGCAAG7800.014.94230651
TCTAACG750.00410657514.82
TATACTG4900.014.7244895
GTACTAG2400.014.6458341
TCTATAC4800.014.6458343
TAAACGC3550.014.59154928
TCTAGCG3700.014.528
GTATAGG4850.014.4948451
CGGACCA7100.014.3309869
GTATCAA130650.014.3298892
TAACGCC4350.014.0344824
CTAGACT3750.013.8133334
Produced by FastQC (version 0.11.5)