FastQCFastQC Report
Thu 28 Apr 2016
ERR1042021.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042021.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3990524
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT207740.5205832617470788No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT145420.3644132950960826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT138260.3464707892998513No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64220.16093124612206317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55550.024.3114321
GTATCAA81350.016.5783652
ACGGACC5850.015.4957268
GTATTAG9200.015.4836941
TCTAGCG3950.014.05063228
CGCAATA4100.013.98780436
CTAGCGG4250.013.92941229
CGGACCA6850.013.7737249
TTAACGG2151.9826984E-1013.76744235
GACGGAC6050.013.7603317
CGTTAGA1254.8502516E-513.322
GCGTAGA1857.398012E-813.01
CGATCGC2850.012.98245619
ATCAACG104350.012.8179214
TAGACAG6750.012.6074085
AACGCAG105950.012.606897
TCAACGC106300.012.5827855
TAGTACG2501.6007107E-1012.584
TATCAAC107950.012.5275583
TATACTG8300.012.4819285
Produced by FastQC (version 0.11.5)