FastQCFastQC Report
Thu 28 Apr 2016
ERR1042020.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042020.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3716786
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT218920.5890035100218307No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT173030.4655366222322189No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT156890.4221120075247808No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63150.16990485866014346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG801.618255E-518.510
GTACTAG2500.018.51
GGTATCA79500.017.8716981
GCGTTAT5300.015.0094341
TAAACGC6050.014.67768628
GTAAACG6100.014.55737727
GTATATG5350.014.1775711
GTATTAG10050.013.990051
ACGGACC6550.013.8396958
ACGCTTC6300.013.80158831
GTGTAGA6600.013.7348481
TTAGCGA1356.575954E-613.70370327
CTAGCGG5300.013.61320829
CGTTATT4500.013.5666672
GACGGAC6750.013.429637
TCTATAC4850.013.3505153
CGCAATA5700.013.30701836
CGAACGA2800.013.21428516
TATACTG6600.013.1742425
CGCAAGA7850.012.9617832
Produced by FastQC (version 0.11.5)