FastQCFastQC Report
Thu 28 Apr 2016
ERR1042019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3231779
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT171940.5320289537124908No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT132230.4091554527707495No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT123890.3833492327290944No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45790.14168666855004627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43700.026.7128141
TAAATCG500.00703757218.59
GTATCAA69550.016.7311292
ACCGTCG3000.016.0333338
ACGTCGA700.002593856315.85714328
TACCGTC3300.015.696977
GTATTAG6750.015.3481481
CGAACGA2450.015.10204116
CGTCGTA3000.014.810
ATACCGT4450.014.5505636
GTGTAAG6900.014.478261
TATACTG6150.014.4390245
GACGGAC4850.014.1134027
TCTAGCG3700.014.028
ACGGACC4950.013.8282838
GTAGCAC2950.013.796613
CTAGCGG3650.013.68493229
AAGACGG5950.013.6806725
CGCAATA3800.013.63157936
CCGTCGT3450.013.4057969
Produced by FastQC (version 0.11.5)