FastQCFastQC Report
Thu 28 Apr 2016
ERR1042018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3725266
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT181840.4881262170271868No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT138540.3718929064394328No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT127890.3433043439045695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49780.13362804159488206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51300.022.4307981
GACGGAC5700.016.8771937
ACGGACC5850.016.4444458
CGCAAGA6650.015.5789472
GTATAGA6450.015.20155051
AAGACGG7000.014.85
GTATCAA78400.014.65372
CGCAATA4050.014.61728436
TCTAGCG3550.014.59154928
CTAGCGG3750.014.30666729
GCGCAAG7250.014.2896561
TACGACG3250.014.2307695
TATACTG6750.013.9777785
CCTATAC6300.013.5079373
CGAGCCG6200.013.42741915
ATACCGT5100.013.4215696
CGGACCA7200.013.3611119
AACGACG850.00940990313.0588247
TTAACGG1703.7377686E-713.05882435
GCGAAAG7000.012.95000118
Produced by FastQC (version 0.11.5)