FastQCFastQC Report
Thu 28 Apr 2016
ERR1042017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2193060
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT126190.5754060536419432No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT95090.43359506807839276No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86440.39415246276891647No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32740.1492891211366766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40550.019.2527751
CGTCGAG902.154029E-618.510
TGCGTAA500.007036871318.49999810
CGCAATA2000.016.6536
ACTATCG700.002593499615.8571438
TAATACT4400.015.5568184
CTAGTAC2400.015.4166663
TATACTG3850.015.3766235
TAGGTCG1701.4879333E-915.23529421
GGACCGT855.366908E-415.2352946
TAGTACT3400.015.2352944
CTAGACA2500.014.8000014
CTATCGC750.00410598514.89
GTCGTAC750.00410598514.81
TATACAG5100.014.5098055
CGTATTA908.2782365E-414.38888915
ATCCGTA908.2782365E-414.38888912
CCTAGAC3500.014.2714293
CATTGCG1854.9058144E-914.029
CCGTCGA1203.3030465E-513.8749999
Produced by FastQC (version 0.11.5)