FastQCFastQC Report
Thu 28 Apr 2016
ERR1042016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5510546
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT448430.8137669116635629No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT339650.6163636053487259No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT316440.5742443670736076No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125470.22769068618608757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148950.019.822761
TCTAGCG6200.016.70967928
CTAGCGG6500.016.50769229
CGAACGA6400.016.47656216
ACGGACC8600.015.0581398
ACGAACG7600.014.36184215
CGCAATA7400.014.2536
TTAACGG2750.014.12727235
TAACGGC2900.014.03448336
GTATCAA210400.013.98930552
TACGACG5450.013.9174315
TACCGTC6550.013.8396957
AAGACGG9800.013.7806135
GTATTAG14800.013.751
TCCGATA8100.013.7037048
TCTATAC7050.013.64539053
CGAACTA3000.013.56666729
CGTCGAA1102.4590135E-413.45454529
TAACGAA8150.013.39263913
GATAACG8300.013.37349411
Produced by FastQC (version 0.11.5)