FastQCFastQC Report
Thu 28 Apr 2016
ERR1042015.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042015.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719403
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT695962.559238185734148No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT510591.8775812191131656No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT474311.744169584280079No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185010.6803331466502023No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71190.2617853992218145No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61050.2244978033781679No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC53340.19614599233728874No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG52600.19342480684179578No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT51180.18820307251260662No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50680.18636443366430058No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50010.1839006576075705No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA38410.14124423632687028No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36860.13554445589712152No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA36310.13352195316398488No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG31570.11609165688204359No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA31270.11498847357305995No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29770.10947255702814183No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA28070.1032211849439013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCGC951.6782178E-719.47368410
GGTATCA262350.016.4938071
TACGACG2700.016.4444455
TCTAGCG1403.4786353E-815.85714228
ACGGACC2100.015.8571428
GCGTTAT2250.015.6222221
GACGGTA2650.015.3584918
CTAGCGG1455.3538315E-815.31034529
CTTAGAC2251.8189894E-1214.83
GTGCGCT1252.9621606E-614.7999999
GTCTAAC1904.5656634E-1014.6052641
GCGCAAG2800.014.5357141
GCGCTAT1406.003047E-714.53571411
TCTAACG1152.2119286E-514.4782612
ACGACGG3600.014.3888886
TAGTCCG1051.6569441E-414.0952385
ACGGTAT2900.014.0344839
CGGTCCA1854.9076334E-914.010
TCGCGCA800.00630153713.87500125
CGTGCGA950.001245838913.63157910
Produced by FastQC (version 0.11.5)