FastQCFastQC Report
Thu 28 Apr 2016
ERR1042014.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042014.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3080876
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT243100.7890612929569382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT176080.5715257608550296No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT155300.5040774117491259No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64690.20997274801063076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81550.018.6701411
TCTATCG609.2416216E-418.531
GTACTAG1851.8189894E-1116.01
TTAACGG2109.094947E-1214.976191535
TTAGCGA1252.9624698E-614.79999927
ATCCGTA1252.9624698E-614.79999912
ACGGACC2900.014.6724148
GCGTTAG908.27958E-414.3888881
CGAACGA2450.014.34693816
TAACGAA2650.013.96226413
TACTCCG4000.013.8750015
GTACCGA800.00630179113.8756
TTAGACT3750.013.8133334
GACGGAC3250.013.6615387
TATACCG1907.1395334E-913.6315795
TAACGCC3000.013.5666674
GTATCAA112450.013.5068922
TAACGGC2355.638867E-1113.382977536
TAATACT6250.013.324
ATAACGA2651.8189894E-1213.26415212
Produced by FastQC (version 0.11.5)