FastQCFastQC Report
Thu 28 Apr 2016
ERR1042013.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042013.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2357191
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT135910.5765761026577821No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT101950.4325063179012647No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT89750.3807497992313733No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29980.1271852811248643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35700.024.3039231
TAGACCG705.1056777E-621.1428595
ACGTTCG609.240743E-418.57
GTATTAG7100.018.2394371
CTAGTAC1900.017.5263163
TAACGCC1800.017.4722234
CGTTCGA650.001580599917.0769238
ATAACGC2100.016.7380963
TTAGACT2450.016.6122444
TTAGGAC4400.016.3977283
TATACTG3500.016.3857145
GACGGAC1851.8189894E-1116.07
GTATCAA54300.015.9788222
TAATCGA4300.015.91860421
TCTATAC2950.015.6779663
ATCGATA4500.015.62222223
TAGTACT2850.015.5789484
GTAATCG4550.015.45054920
AATCGAT4550.015.45054922
ACGACCA3500.015.32857111
Produced by FastQC (version 0.11.5)