FastQCFastQC Report
Thu 28 Apr 2016
ERR1042012.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042012.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3009869
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT199310.6621882879288102No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT151210.5023806683945381No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT130490.4335404630566978No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44720.14857789491835027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73450.017.5051061
CTAACGC1059.3530725E-615.8571423
AGTGCGC1557.221388E-915.516129510
TAGACAG4800.015.0312515
TTAACGG1508.1146936E-814.835
TATTCCG1406.0036655E-714.5357145
GTAGCAC2650.013.9622643
AGCGTTA800.006301746713.87510
GCGTTAT2700.013.7037041
GTCTAAC2051.4260877E-913.5365851
ACGGACC4100.013.5365858
GACGGAC4050.013.2469147
TAACGAA2950.013.16949213
GTAAACG3100.013.12903227
CGAACGA2703.6379788E-1213.01851816
TATACTG4550.013.0109885
CGGACAT5700.012.98245721
GTCTTAC2850.012.9824571
TCTATAC3450.012.8695653
TTTAGCG1153.5801655E-412.86956426
Produced by FastQC (version 0.11.5)