FastQCFastQC Report
Thu 28 Apr 2016
ERR1042011.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042011.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10616279
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT599170.5643879555162407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT386210.36379036383651936No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT370920.34938795410331625No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170690.1607813811223311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA190550.021.0194171
ACGGACC20350.015.9090918
TCTAGCG12400.015.81451528
GACGGAC20150.015.607947
CTAGCGG13450.014.85501929
GTATCAA274350.014.5990512
AAGACGG23000.014.3173915
CGCAATA14150.014.25088436
CGGACCA23650.013.8456669
TACGACG14400.013.6180565
GCGCAAG25900.013.5714271
CGCAAGA24350.013.2956892
CAAGACG28150.012.8809954
TAGTACG3800.012.6578954
CTAGACA12900.012.6201554
TAGACAG16750.012.4805975
CGACGGT16050.012.4485997
AGACGGA25600.012.4296886
GCGTTAT15250.012.3737711
GCGAAAG25300.012.35770818
Produced by FastQC (version 0.11.5)