FastQCFastQC Report
Thu 28 Apr 2016
ERR1042010.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042010.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7669280
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT526940.6870788392130682No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT376250.49059364112406906No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT342440.44650866834957126No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150890.196745978762022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA163750.022.346871
GTATCAA232450.015.7184342
ACGGACC9000.015.4166678
GACGGAC9300.014.3225817
TCTAGCG6800.014.14705928
CGCAATA7500.013.81333436
GTACCGT1102.4592105E-413.4545456
AAGACGG11950.013.1589975
CTAGCGG7350.013.08843629
AACGCAG290450.012.554147
GCGTTAT7100.012.2464791
ATCAACG298700.012.244564
TTAGGAC9050.012.0607733
TCAACGC303700.012.0246965
TATCAAC306650.012.017613
CGGACCA12050.011.9751039
TAATACT13600.011.9705894
TATACTG13050.011.9080475
CAACGCA308400.011.8234446
TCTATAC9400.011.8085113
Produced by FastQC (version 0.11.5)