FastQCFastQC Report
Thu 28 Apr 2016
ERR1042009.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042009.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4622869
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT375400.8120498331231104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT257850.5577705100447363No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT240480.5201964407816877No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100690.2178084648299573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121050.020.204051
GACGGAC7250.017.3517237
CGTATAC1107.820163E-716.8181823
ACGGACC7350.016.6122468
TCTAGCG4700.015.35106528
CCGTATA855.368508E-415.2352942
CGGACCA8300.015.1566279
AAGACGG9550.014.5287975
GTATCAA173200.014.1420342
CGAACGA4900.013.96938716
CGTCGAA800.006302417313.87500129
CGCAAGA9850.013.710662
TCTAACG1356.57678E-613.7037042
CTAGCGG5300.013.61320829
GCGCAAG10000.013.5051
TATACCG1805.1701136E-813.3611125
AGACGGA9900.013.2676776
GTATAGA5550.013.01
GTATATA8300.012.9277111
CAAGACG11600.012.9181034
Produced by FastQC (version 0.11.5)