FastQCFastQC Report
Thu 28 Apr 2016
ERR1042008.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042008.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7194250
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1133031.574910518817111No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT864171.2011953991034507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT691400.9610452792160405No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267490.37181082114188413No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT81760.11364631476526393No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79140.11000451749661189No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA75270.10462522153108385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC12350.017.0769238
GACGGAC12400.016.8588717
GGTATCA418650.016.2794691
CGCCACA14100.015.744681
GCGAAAG13450.015.26765918
CGGACCA13700.015.1240879
GCGCAAG15700.014.8471341
CGCAAGA14600.014.57191852
CGCTATA1152.2129303E-514.478262
AAGACGG16750.014.3582095
AGCGAAA14500.014.03448217
TACCGTC7700.013.9350647
TATACTG11850.013.7383975
ACAACAC36800.013.6739123
CGAACGA6900.013.67391216
AGACGGA15100.013.5993396
GAGCGAA15000.013.56666616
CAAGACG18600.013.4274194
CGAAAGC15150.013.31023119
CGCTTCG6400.013.29687532
Produced by FastQC (version 0.11.5)