FastQCFastQC Report
Thu 28 Apr 2016
ERR1042007.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042007.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11757740
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT882020.750161170429011No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT593350.5046463010748664No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT555510.4724632454876533No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271640.23103079333273233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA300400.017.7733021
TCTAGCG10900.016.12385428
CTAGCGG12100.014.83057929
ACGGACC17350.014.6080688
CGCAATA13800.013.27173936
GTATCAA408100.013.0692232
GACGGAC18800.012.9893617
TATACTG20300.012.8497535
CGGACCA20600.012.5728169
CGAACGA11400.012.33333316
TAACGCC13800.012.1992764
AAGACGG22450.012.1959915
CGAGCCG16750.012.14925415
AACGCAG444000.012.0166667
CGCAAGA23050.011.9587852
CGGTCCA16450.011.80851110
TACCCCG6150.011.7317085
ACGAACG11950.011.61087815
GTATAGA16900.011.603551
GTACATG471150.011.5833611
Produced by FastQC (version 0.11.5)