FastQCFastQC Report
Thu 28 Apr 2016
ERR1042006.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042006.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15392105
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1212330.7876310615084812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1212280.7875985773225949No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT919260.5972282543550737No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365180.23725150003849377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA442050.019.5692791
TAATACT54100.013.6099824
GTATCAA652200.013.2665592
TCTAATA38600.013.0841972
TTAACGG24400.012.88934435
GGGTTAG30500.012.677057
GGGGTTA30150.012.6401336
TATTAGA34300.012.513122
GTATTAG63250.012.3723321
ATTAGAG35750.012.160843
AATACTG52700.011.830175
TTAGGAC24150.011.5672873
TAACGGC27000.011.37407436
ATACTGG38800.011.3479396
TACTGGT35100.011.2792027
CTCTTAT118000.011.20974637
GTACTAT22450.011.2071281
ATAGTAC28750.010.9391313
AACGCAG837000.010.9209685
GTATAGG44750.010.9139661
Produced by FastQC (version 0.11.5)