FastQCFastQC Report
Thu 28 Apr 2016
ERR1042005.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042005.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12874336
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1264370.9820856003758174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1227920.9537734606274063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT933620.7251791471031982No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359060.2788959368467624No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT141290.10974546570790136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA547550.015.9271311
TTAACGG18000.015.10833435
TAACGGC21150.013.12056636
TATTAGA27750.012.9333332
TCTATAC10900.012.7293583
TAATACT38550.012.2373544
GGGGTTA21900.011.9954356
ACACCGT10500.011.9809526
ATTAGAG29450.011.9354833
GTATTAG49350.011.8459981
TCTAATA31600.011.7674052
GTATCAA750250.011.6338562
CGAACTA22200.011.524
TAGGGGT20600.011.405344
AATACTG38300.011.3511745
TATACAG16150.011.1114555
CTCTTAT85550.011.07188937
TATACTG16050.011.065425
TATCTAG23600.011.0529661
TAGGACC25450.010.9764244
Produced by FastQC (version 0.11.5)