FastQCFastQC Report
Thu 28 Apr 2016
ERR1042004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13157677
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT596450.45330950136562864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT578030.43931006970303343No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT443130.3367843731078062No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164620.12511327037439815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA285950.018.1085851
TTAACGG23350.015.68736635
TAACGGC25450.014.32023636
TATTAGA34750.014.2676272
TAATACT42350.013.8040134
ATTAGAG34350.013.7336243
GTATTAG58900.013.2546691
TCTAATA31850.012.894822
AATACTG38550.012.8132295
GTATCAA406400.012.7324072
GGGGTTA23550.012.3333336
GTACTAT20300.012.21182251
CGAACTA28400.012.18133824
TTAGGAC22400.011.56253
TTTAACG34000.011.53529534
GGGTTAG26000.011.3846157
GCGAACT30800.011.29220823
GTGACAC35800.011.26536424
ACCGCCT35550.011.24050612
GGCACCG35500.011.20422659
Produced by FastQC (version 0.11.5)