FastQCFastQC Report
Thu 28 Apr 2016
ERR1042002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12884425
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT840840.6526018817292972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT791020.613935041726736No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT610400.4737502837728498No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230290.1787351783257693No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT184540.14322719096894118No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG130610.10137045308579933No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG129940.10085044540210371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA12500.014.94826
GTACTAG13450.013.75464630
CGTACTA13600.013.73897129
ACTAGAT14450.013.44290732
TTAACGG18050.013.324135
TCTAATA28750.013.1269562
TAATACT36000.013.1041674
GGGGTTA25950.012.9749526
GGTATCA513050.012.4619441
TACCCCG17250.012.3333345
GGGTTAG27500.012.1763647
GTACTAT17100.011.7923971
AATACTG36250.011.7889655
CTTACCC18250.011.7589043
TATTAGA28500.011.684212
TAGGGGT23000.011.6630444
TAACGGC20250.011.60246936
TCTAGCG6100.011.524590528
CTAGATA20850.011.3573143
CTAGATC17200.011.29360633
Produced by FastQC (version 0.11.5)