FastQCFastQC Report
Thu 28 Apr 2016
ERR1042001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15936341
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT887800.5570914929593939No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT876810.5501953051832914No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT658190.4130119956645004No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228340.14328257659647217No Hit
TATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACTAT171920.10787921769495269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA453900.019.1317481
GCGAATC16450.015.96960533
GGCGAAT18300.014.55737632
TAATACT53550.014.2679734
GACTAAG18950.014.05804626
TATACTG15800.013.9335445
TTAACGG34000.013.82058835
TCTAATA41100.013.6836992
TATTAGA45650.013.4950712
AATACTG47450.013.1390935
GTATCAA662800.013.099052
ATTAGAG43350.013.0588243
GTACTAT22600.013.0154861
TAACGGC36300.012.89393936
AGACTAA20850.012.6882525
TACTGGT40000.012.2562497
ATGCTAG32700.012.16360915
ACTAAGG22450.012.0311827
ATACTGG42200.012.01184756
CTCTAAT42800.011.973131
Produced by FastQC (version 0.11.5)