FastQCFastQC Report
Thu 28 Apr 2016
ERR1042000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11429936
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1129470.9881682627094326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1064180.9310463330678318No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT797910.6980878983049424No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291760.25525952201307167No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT220450.19287072123588442No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG213030.18637899634783606No Hit
TATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACTAT207240.18131335118586842No Hit
GTATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACTA169870.1486185049505089No Hit
GGTATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACT161870.1416193406507263No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG152530.1334478163307301No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT143680.1257049908240956No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG135440.11849585159531952No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG128120.11209161626101845No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG123940.10843455291438202No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT116830.10221404564295024No Hit
ATACTAAAGTTTGATTAGCCAATAGATTCCTGTGGGGAAAAAA116150.1016191166774687No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT115090.10069172740774751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA716000.012.8673181
TACACAT22650.012.2516565
CACGAGA18600.012.03494621
CTTATAC43850.011.93956737
CTAGATA20250.011.8765433
ATACCGT10400.011.56256
TACCGTC7550.011.5165567
TATACTG12550.011.4980085
TCTAGAT23100.011.4523812
ACACCGT10350.011.4396136
TCTAATA10350.011.4396132
TAATACT13850.011.353794
ACGAGAC20250.011.23703822
TATACCG3650.011.1506855
TAAACGC11150.011.11659128
TAGACAG11000.010.9318195
CGCTATA5300.010.8207552
TAAGACT8700.010.6321834
GTAAACG12450.010.550227
ATACACA27100.010.5129154
Produced by FastQC (version 0.11.5)