FastQCFastQC Report
Thu 28 Apr 2016
ERR1041999.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041999.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10075181
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT837720.8314689334117173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT819300.8131863834505803No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT568860.564615166715119No Hit
TATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACTAT370270.36750704528285894No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT326790.3243514930401747No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG299890.29765222083851395No Hit
GTATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACTA297420.2952006519783615No Hit
GGTATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACT271660.2696328730967712No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG227600.22590164881405106No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT220000.2183583600135819No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219960.21831865849357945No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG208180.20662656085285216No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG201630.20012543695244778No Hit
GTATATAGTCAAGATCTGGGGGAGATGACCCGTACTCTGCGTT192680.19124222185189527No Hit
CTTTAGTATATAGTCAAGATCTGGGGGAGATGACCCGTACTCT183090.18172378243130322No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG175230.17392243375081798No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT174310.17300929879076118No Hit
TTAGTATATAGTCAAGATCTGGGGGAGATGACCCGTACTCTGC143120.14205203856883564No Hit
CTATTGGCTAATCAAACTTTAGTATATAGTCAAGATCTGGGGG137620.1365930795684961No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT131370.13038971706811023No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG112220.111382614366928No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA105820.1050303711665329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAT34050.017.3318655
CTCTCTA39250.015.55413929
TCTACAT40050.015.01248432
ATACACA41800.014.7822964
CACGAGA41200.014.77305921
ATCTCCG42150.014.6595510
TACATCT41400.014.61231834
TTTGCGT18650.014.2841823
CTCTACA42600.014.1138531
ACGAGAC43000.014.06860522
CCGAGCC48100.014.03846214
CTACATC43300.013.97113233
CACATCT45900.013.9455337
TCCGAGC44700.013.86465413
ATTACAT26300.013.6463873
CGAGCCC44350.013.64036115
GCCCACG44700.013.574944518
ACCTCTC45500.013.5395627
GACCTCT45500.013.417582526
TCTCCGA45800.013.37008811
Produced by FastQC (version 0.11.5)