FastQCFastQC Report
Thu 28 Apr 2016
ERR1041998.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041998.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7993433
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT343410.429615160344748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT318830.3988649182397601No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT207000.2589625759044956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123150.022.5184751
GTATCAA176250.015.6921992
TTAACGG13000.014.80000135
TAACGGC14250.013.24210536
TATTAGA20400.013.0588242
GTCGGGA15100.012.8642392
ATTAGAG19150.012.5587463
AATACTG20200.012.547035
TAGGGGT13250.012.4264154
GTATTAG37550.012.4154451
GGGGTTA14200.012.2464796
GGGTAAG10700.012.1028041
AAACGGA23150.012.06695432
AACGGAG23600.011.99364533
TCTAATA17600.011.9829542
CTAGATA14250.011.9438593
TAATACT25750.011.9262144
AACGCAG258850.011.8997495
TGCGTCA9650.011.88601110
GTTCTAG10750.011.8744181
Produced by FastQC (version 0.11.5)