FastQCFastQC Report
Thu 28 Apr 2016
ERR1041997.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041997.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8817768
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT467690.530394993381545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT436230.49471703043219106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT273980.31071355018639635No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115950.1314958615377497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144700.022.5912231
GTATCAA200400.016.2659682
TTAACGG12350.013.48178135
GGGGTTA17800.012.887646
TAATACT28800.012.8472224
TTAGGAC16600.012.4819283
GTATTAG35500.012.090141
TAGGACC19000.012.0736844
TCTAATA21300.011.8990612
AATACTG27800.011.8453245
TATACTG11850.011.708865
GGGTAAG11700.011.7008541
AACGCAG285900.011.6991955
ACACCGT7250.011.48275856
GGGTTAG19400.011.4432997
CCGAGTT9750.011.38461513
CTCTAAT23700.011.2405061
GTATAGA10400.011.2067311
GCCTAAG21650.011.1939951
ATAGGGG15400.011.1720773
Produced by FastQC (version 0.11.5)