FastQCFastQC Report
Thu 28 Apr 2016
ERR1041995.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041995.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7962070
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT271280.3407154169707124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT231080.29022603418457765No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT157340.19761192755150359No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT142310.1787349269725084No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG119860.1505387418096048No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG104360.13107144247664237No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT87300.10964485366242699No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG82270.10332740104018177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCCG9350.014.6417125
GTCGGGA14700.014.3469382
TAATACT19850.012.8614614
TATTAGA16400.012.6341472
TTAACGG12650.012.28458535
TATACTG11000.012.2772735
ATTAGAG17600.011.9829543
CGAACTA14550.011.9518924
GTACTAT12900.011.9031011
GGGGTTA15900.011.7515746
GCTATAC7750.011.69677453
ATACCGT6500.011.669236
TTATGCG9900.011.5858584
GTATTAG33700.011.5830851
ACCGTCG4800.011.5625018
GTATACG1603.7692113E-511.56251
GGGTAAG10400.011.3846151
GGGTTAG17100.011.3596497
AATACTG20950.011.3031035
CTAGACT6900.011.260874
Produced by FastQC (version 0.11.5)