FastQCFastQC Report
Thu 28 Apr 2016
ERR1041994.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041994.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9417106
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT508960.5404632803326201No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT469680.49875195203282197No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT344840.36618468561360573No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146290.15534496479066925No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT131990.1401598325430339No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG121380.1288931015537045No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG94890.10076344048798005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG15400.013.33441535
TATTAGA21150.012.6832142
GTCGGGA20250.012.6074082
TAATACT29300.012.5648464
AATACTG26950.012.1502795
TCTAATA23650.012.1247372
GTATTAG43150.012.0903821
TAACGGC17700.011.70621436
TTAGACT8400.011.6726194
GGGGTTA18100.011.6519346
TAACGCC4300.011.6162794
ATTAGAG22650.011.5982353
GTAAACG11050.011.55203627
TATACTG10200.011.4264715
TTAGGAC16150.011.3405573
GGTATCA350150.011.1533631
ATGCTAG20600.011.135921515
TAGGACC19100.010.9450274
TAGGGGT18100.010.9364644
GGGTTAG20050.010.887787
Produced by FastQC (version 0.11.5)