FastQCFastQC Report
Thu 28 Apr 2016
ERR1041992.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041992.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8427652
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT152750.18124858501513827No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG136820.1623465230885186No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT127530.15132328672327713No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC111610.13243309049780413No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC110480.13109226626823223No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC105670.12538486401669172No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA105330.1249814301777055No Hit
TATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACTAT97840.11609402001886172No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG96790.11484812139846307No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT96770.11482438999616976No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC93330.11074258880172082No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA89220.10586578563044607No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT89030.10564033730865964No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT85870.101890775746317No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC85820.10183144724058373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAT27100.018.2269365
GCCGTCT10800.017.47222336
TATACAC25950.017.466283
GTCGGGA42700.017.2868842
ATCTCCG34100.015.84164210
ACATCTC35450.015.8124118
TTAACGC1302.594461E-715.65384635
ATACACA32650.015.4119444
ACACATC37350.015.4042846
TATTAGA20000.014.9852
TCCTAAC32000.014.6265632
GGGAGTG59150.014.6060875
GGTCGGG52100.014.5585421
CGCTATA3850.014.4155842
TTATACA31600.014.0506322
CATGTGG53550.013.7843141
CGTCTAA2700.013.7037041
TCTCCGA39650.013.6708711
ATGCCGT13950.013.65949934
ATCTCGT39950.013.475594537
Produced by FastQC (version 0.11.5)