FastQCFastQC Report
Thu 28 Apr 2016
ERR1041991.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041991.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11706763
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT712780.6088617323166106No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG660340.564067112317897No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG484030.4134618596105516No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG469970.40145170787176615No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT461880.39454117248294857No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG452840.38681914035502385No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT403450.3446298519923911No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG379930.3245389011462861No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG227320.1941783565619292No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA219820.18777180335845187No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCG196890.16818483469768714No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTG192030.16403338822183383No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGC186400.15922420228375683No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT178900.15281764908027948No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT175740.15011835466388104No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTA172780.14758990166624197No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA169490.14477956032764994No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT166760.14244757496158417No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC165630.14148232094559357No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC164960.1409100021927496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT160380.13699773370315946No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA157490.13452907520208618No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG156110.13335026941264636No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT152530.13029220801685318No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC139660.11929856271968606No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC136360.11647967931015601No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG132670.11332765513404518No Hit
TATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACTAT129800.11087608077484784No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC128670.10991082675885724No Hit
ACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC121340.10364948876132539No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT120280.1027440292419006No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAG120170.10265006646158292No Hit
GTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGA119950.10246214090094759No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCA119450.1020350373540491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGGA52450.015.061012
TTAACGG12800.014.742187535
TATTAGA18650.013.2922262
GGTCGGG61500.013.0252031
GGGAGTG73700.012.9525115
TCTGTCG17900.012.9189958
GTATAGA6800.012.7867641
TATAACG1602.7008155E-612.718752
CGGGAGT82500.012.6472734
TAACGGC14700.012.45918436
GTGGGTA66900.012.1950679
TATACTG7400.012.05
CTCTTAT62950.011.96108137
TAATACT15600.011.85897354
TGGGTAA68450.011.78378410
GTATTAG39550.011.6472831
TCGGACG16850.011.63798113
CATGTGG58650.011.6078421
TATACAC23000.011.5021743
TACACAT25000.011.3965
Produced by FastQC (version 0.11.5)