FastQCFastQC Report
Thu 28 Apr 2016
ERR1041990.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041990.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2228693
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT87550.3928311346605387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT77030.34562858141520614No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56500.2535118116313014No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23490.10539809655255344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26950.025.7421151
GTATCAA41850.016.5328562
TGCGACG803.3843477E-416.187522
GAACCGT803.3843477E-416.18756
ACGAACG1508.1114194E-814.829
TCTTATA25650.014.49707637
CGAACTA4850.014.49484524
GTACGTA2301.8189894E-1214.47826113
TACCGAC1551.2116288E-714.322587
ATACCGT1051.6567322E-414.0952386
GCGAACT5050.013.92079223
GATCGGA800.006301067313.8750011
GGTCTAA800.006301067313.8750011
TTTAGCG1356.5731256E-613.70370326
ATACCGA1356.5731256E-613.7037036
TACCCGC2051.4260877E-913.53658611
CTCTAAT5550.013.3333331
GGCACCG6400.013.2968769
TACCCCG4500.013.1555565
ATAGACC850.00940835413.0588244
Produced by FastQC (version 0.11.5)