FastQCFastQC Report
Thu 28 Apr 2016
ERR1041989.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041989.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12383347
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT219900.17757719298344785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT187470.15138879658302395No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT166180.13419635256930132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69700.026.064561
TCTTATA182550.016.63023837
CTTATAC111400.016.29129237
GTATCAA119900.015.1363652
CTCTTAT302150.014.21098837
GTATTAG43400.013.55531
TTAACGG20300.013.39655235
TAACGGC20650.012.99031536
ATTAGAG29850.012.7051933
TAATACT47800.012.4236394
TCGTTCG2255.7243597E-912.33333323
TATTAGA37850.012.3170412
TACCCCG17300.012.29768855
TTTAACG23550.012.25477734
AATACTG41100.012.1532845
ATCAACG174700.012.0509442
CTAATAC43850.011.9817553
TCTATAC15650.011.8210873
TCAACGC179000.011.7821233
CGAACTA19550.011.73401524
Produced by FastQC (version 0.11.5)