FastQCFastQC Report
Thu 28 Apr 2016
ERR1041988.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041988.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8860066
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT93940.10602629822396357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37450.024.9959931
CTTATAC60650.016.50206237
TCTTATA106300.016.18532437
GTATCAA66500.015.4954891
CTCTTAT163000.013.08619537
TTAACGG8650.011.97687935
ATCAACG92050.011.9179782
TTAGAGG19100.011.9136134
TCAACGC93200.011.7907723
TAACGGC8900.011.43258536
TATACTG15050.011.4318945
TCTATAC11050.011.3846163
CAACGCA97800.011.2929454
ATTAGAG15000.011.2233343
AATTTCG8800.011.14204528
TACGCAG2502.7212081E-811.15
TAATACT25200.011.0119054
CGTGCGA2707.195922E-910.96296310
ATAATAC19000.010.9052633
TATTAGA19050.010.8766412
Produced by FastQC (version 0.11.5)