FastQCFastQC Report
Thu 28 Apr 2016
ERR1041987.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041987.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14527600
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT246080.16938792367631267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT211760.14576392521820533No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT198110.13636801674054902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72550.026.060651
CTTATAC112350.016.94392637
TCTTATA185450.015.58209837
GTATCAA124300.015.1512462
GTATTAG49150.013.39979651
TTAACGG20850.013.22062335
TAACGGC21350.012.82435636
CTCTAAT33850.012.7341211
TAATACT58450.012.6920444
CTCTTAT278850.012.49919237
CTAATAC51900.012.36897853
AATACTG47100.012.2940555
ATACTGG43050.012.2044146
TAACCGG9400.012.20212822
ATTAGAG33400.012.13023953
ATCAACG181200.012.04746152
TCAACGC181800.011.9262923
CAACGCA185150.011.7904414
TTTAACG25350.011.67652934
GGTAAAC29500.011.60169535
Produced by FastQC (version 0.11.5)