FastQCFastQC Report
Thu 28 Apr 2016
ERR1041985.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041985.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12051793
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT250330.20771183175814587No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT212490.17631401402264377No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT191990.15930409690906575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78350.026.6343351
CTTATAC93450.015.97592437
TCTTATA148150.015.671617537
GTATCAA132550.015.617882
CTCTTAT231200.013.01081437
GTATTAG34700.013.0086451
TCTATAC14850.012.2087543
ATCAACG194500.012.1748082
TCAACGC198550.012.01032453
ATTAGAG24250.011.8247423
GCGAACT20000.011.74749923
CAACGCA202500.011.7212344
TAATACG8400.011.6726194
TCTGTCG16850.011.528198
TATTAGA31500.011.4523812
TTAACGG15700.011.31210235
AACGCAG212650.011.2835655
TAATACT43650.011.2737684
GTATTAA19000.011.1973691
CGAACTA20850.011.17985624
Produced by FastQC (version 0.11.5)