FastQCFastQC Report
Thu 28 Apr 2016
ERR1041984.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041984.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4563454
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT150910.3306924973934217No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT135730.2974282199404223No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT101400.22220011421173524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61850.020.0703331
TATACCG2100.016.7380945
CTTATAC38400.015.56119837
TCTTATA65800.015.32294937
GTATCAA90500.013.7370172
CTCTTAT102050.013.54189237
CGTATAC1951.0282747E-813.2820523
TATACAC17250.012.86956537
CGAACTA2603.092282E-1012.09615424
TATACTG7750.011.6967745
ATACCGA3259.094947E-1211.3846166
TATTCCG1300.001003921311.3846155
TCTCTTA150150.011.28604737
TATACAG7400.011.255
TACCGAC2655.3678377E-911.169817
ATACCGT3351.4551915E-1111.0447766
TACGGTT1350.001376330410.96296314
TCTATAC4900.010.9489793
ATCAACG125550.010.7714052
TAAAGCG2259.3782546E-710.6888895
Produced by FastQC (version 0.11.5)