FastQCFastQC Report
Thu 28 Apr 2016
ERR1041983.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041983.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10182590
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT683060.6708116500811679No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT548350.5385172141861746No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT494920.48604529888761105No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209570.20581207728092754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA184650.021.9014341
TATACCG4000.017.1125015
CTTATAC134850.017.10752737
TCTTATA222650.015.29687837
GTATCAA291550.013.8329622
TATACAC47000.012.98936137
CTAATAC23800.012.825633
CTCTTAT337300.012.730062537
GTATTAG21550.012.5336421
TATAGTC16300.012.25766855
TAATACT29700.012.0841754
TTATAGT20850.012.0671464
GTACTAG6250.011.5440011
ATCAACG420400.011.4678882
CTCTAAT19800.011.3055551
TCAACGC428700.011.25017453
CAACGCA431400.011.1711864
TATCAAC432000.011.1599531
AACGCAG433750.011.0936015
TCTATAC12500.010.9520013
Produced by FastQC (version 0.11.5)