FastQCFastQC Report
Thu 28 Apr 2016
ERR1041982.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041982.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11084662
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT294830.2659801444554647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT237390.21416079263400184No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT218830.19741693522093862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100950.021.6612191
CTTATAC108100.018.65402437
TCTTATA199850.016.88466337
GTATCAA158250.013.7011052
CTCTTAT306300.012.84671937
TATACAC42000.012.37738137
TATAGTC12500.012.1365
TCTATAC11150.012.1121073
TAATACT21750.012.0781614
TAGACAG15650.011.3482435
TATACCG4250.011.3176475
ATCAACG226400.011.268332
TCAACGC228800.011.1663033
CAACGCA230150.011.1249194
TATCAAC230000.011.0839131
CGCTATA4700.011.02127552
TCTCTTA440650.011.01645437
AACGCAG238950.010.7306965
TTATAGT17200.010.5406974
GTCCTAA14250.010.3859651
Produced by FastQC (version 0.11.5)